The provision of HCMCC services is concentrated around the following aims:
1. Consultation and assistance
Provide consultation and assistance in microbiome sample collection plans development. This includes consultation on collection kit configuration and contents, shipping, processing and storage plan development.
2. Ordering and tracking
Support ordering and tracking of customizable microbiome collection kits. HCMCC kits are mailed through the designated carrier to selected clinical sites or participants. Post collection, kits can be returned through pre-paid shipment to selected labs and biobanks, or BIOM-Mass at Harvard T.H. Chan School of Public Health for sample processing and storage.
3. Sample handling, sequencing and storage
Provide assistance in sample handling, sequencing and storage. Post collection, HCMCC kits can be returned through pre-paid shipment to BIOM-Mass at Harvard T.H. Chan School of Public Health, where a liquid handling robot splits tubes into ~500µl aliquots for -80°C storage operated by BiOS Freezer Core. Aliquots can be retrieved through BiOS LIMS and sent to selected sequencing labs for meta’omics and metabolomic profiling.
Microbiome Sample Collection Kit
The HCMCC kit avoids the need for expensive, bulky, and inconvenient ice packs by providing several different room temperature storage media that are also compatible with multiple different molecular assays including any combination of amplicon (16S), metagenomic, metatranscriptomic, and metabolomics sequencing.
The kit further includes a culture-compatible sample using anaerobic media that yields live microbes for culture or gnotobiotic, as well as user-friendly instructions and standardized questionnaires to capture recent medications, diet, and oral and gastrointestinal health status.
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- Integrative, H.M.P.R.N.C. The Integrative Human Microbiome Project: dynamic analysis ofmicrobiome-host omics profiles during periods of human health and disease. Cell Host Microbe 16,276-289 (2014).
- Franzosa, E.A. et al. Relating the metatranscriptome and metagenome of the human gut. ProcNatl Acad Sci U S A 111, E2329-2338 (2014).
- Schirmer, M. et al. Dynamics of metatranscription in the inflammatory bowel disease gutmicrobiome. Nat Microbiol 3, 337-346 (2018).
- Franzosa, E.A. et al. Gut microbiome structure and metabolic activity in inflammatory boweldisease. Nat Microbiol 4, 293-305 (2019).